TY - JOUR A2 - Sun, Fengjie AU - Tian, Ya AU - Pu, Xiangdong AU - Yu, Haoying AU - Ji, Aijia AU - Gao, Ranran AU - Hu, Yating AU - Xu, Zhichao AU - Wang, Hualei PY - 2020 DA - 2020/04/06 TI - Genome-Wide Characterization and Analysis of bHLH Transcription Factors Related to Crocin Biosynthesis in Gardenia jasminoides Ellis (Rubiaceae) SP - 2903861 VL - 2020 AB - Crocins, enriched in Gardenia jasminoides fruits, have a pharmacological activity against central nervous system diseases, cardiovascular diseases, and cancer cell growth. The biosynthesis of crocins has been widely explored, but its regulatory mechanism remains unknown. Here, the basic helix-loop-helix (bHLH) transcription factors related to crocin biosynthesis were systematically identified on the basis of the genome of G. jasminoides. A total of 95 GjbHLH transcription factor genes were identified, and their phylogenetic analysis indicated that they could be classified into 23 subfamilies. The combination of gene-specific bHLH expression patterns, the coexpression analysis of biosynthesis genes, and the analysis of promoter sequences in crocin biosynthesis pathways suggested that nine bHLHs in G. jasminoides might negatively regulate crocin biosynthesis. This study laid a foundation for understanding the regulatory mechanism of crocin biosynthesis and the improvement and breeding of G. jasminoides varieties. SN - 2314-6133 UR - https://doi.org/10.1155/2020/2903861 DO - 10.1155/2020/2903861 JF - BioMed Research International PB - Hindawi KW - ER -