Protective Effects of Crocetin against Radiation-Induced Injury in Intestinal Epithelial Cells

Figure 1

In vitro experiment design. IEC-6 cells were exposed to 10Gy of radiation, followed by treatment of different doses of crocetin (0.1 μM, 1 μM, 10 μM, and 100 μM) for 24 h, and culture supernatants were collected for CCK-8 assay from day 1 to day 7 after radiation. Apoptosis was detected on days 1, 3, 5, and 7 after radiation. The levels of SOD, CAT, GPx, MDA, MPO, TNF-α, IL-1β, and IFN-γ in culture supernatants were measured on days 1, 3, 5, and 7 after radiation. SOD: superoxide dismutase; CAT: catalase; GPx: glutathione peroxidase; MDA: malondialdehyde; MPO: myeloperoxidase; TNF-α: tumor necrosis factor-α, IL-1β: interleukin-1β, IFN-γ: interferon-γ; IR: irradiation group.