Protective Effects of Crocetin against Radiation-Induced Injury in Intestinal Epithelial Cells

Figure 4

Crocetin ameliorated apoptosis in irradiated IEC-6 cells. (a) Apoptosis of IEC-6 cells was detected by flow cytometry after Annexin V/PI staining 3 days after radiation. The left upper quadrant contains necrotic cells (%); the upper right quadrant contains late apoptotic cells (%); the lower left quadrant contains live cells (%); and the lower right quadrant contains early apoptotic cells (%). (b) The percentage of total apoptotic cells and dead cells was calculated on day 3 after radiation. (c) Apoptotic ratio of IEC-6 cells was detected by Annexin V/PI double staining at 1, 3, 5, and 7 days after radiation. Data were expressed as of three independent experiments. compared to the control group, compared to the IR group. IR group: irradiation group, IEC-6 cells exposed to 10 Gy of radiation. Control group: IEC-6 cells without irradiation or crocetin.

(a)

(b)

(c)