Research Article

Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression

Figure 3

Functional study of miR-150-5p in H9C2 cells. (a, b) Apoptosis was detected by Annexin V-FITC/PI staining (a) and TUNEL assay (b) after transfection. Green represents TUNEL positive cells and blue represents cell nucleus. (a, b) Caspase3, Bax, and Bcl-2 mRNA (c) and protein expression (d) were measured by qPCR and western blot after transfection. I: control, II: LPS, III: LPS+NC, IV: LPS+mimics. Data are presented as , repeated for three times. compared to the control or the LPS+NC groups.