The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An In Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces

Table 1

Data sheet of the specific genes evaluated in this study.

Gene (ID)	Primer	5-3 sequence	Reaction’s condition	Product size (bp)
Integrin b1 (16412)	Forward	CTG ATT GGC TGG AGG AAT GT	95°C, 15 s; 63°C, 30 s; 72°C, 30 s	173
	Reverse	TGA GCA ATT GAA GGA TAA TCA TAG
Fak (14083)	Forward	TCC ACC AAA GAA ACC ACC TC	95°C, 8 s; 61°C, 8 s; 72°C, 8 s	101
	Reverse	ACG GCT TGA CAC CCT CAT T
Src (17977)	Forward	TCG TGA GGG AGA GTG AGA C	95°C, 8 s; 61°C, 8 s; 72°C, 8 s	134
	Reverse	GCG GGA GGT GAT GTA GAA AC
Cdk2 (12566)	Forward	TAC CCA GTA CTG CCA TCC GA	95°C, 15 s; 60°C, 30 s; 72°C, 30 s	466
	Reverse	CGG GTC ACC ATT TCA GCA AA
Cdk4 (12567)	Forward	TCG ATA TGA ACC CGT GGC TG	95°C, 15 s; 60°C, 30 s; 72°C, 30 s	904
	Reverse	TTC TCA CTC TGC GTC GCT TT
Cdk6 (12571)	Forward	CGC CGA TCA GCA GTA TGA GT	95°C, 8 s; 61°C, 8 s; 72°C, 8 s	325
	Reverse	GCC GGG CTC TGG AAC TTT AT
Runx2 (12393)	Forward	GGA CGA GGC AAG AGT TTC A	95°C, 15 s; 63°C, 30 s; 72°C, 30 s	249
	Reverse	TGG TGC AGA GTT CAG GGA G
Osterix (170574)	Forward	CCC TTC CCT CAC TCA TTT CC	95°C, 15 s; 63°C, 30 s; 72°C, 30 s	424
	Reverse	CAA CCG CCT TGG GCT TAT
Caspase1 (12362)	Forward	TGA AAG AGG TGA AAG AAT T	95°C, 15 s; 63°C, 30 s; 72°C, 30 s	385
	Reverse	TCT CAA GAC ACA TTA TCT
Alp (11647)	Forward	GAA GTC CGT GGG CAT CGT	95°C, 15 s; 63°C, 30 s; 72°C, 30 s	347
	Reverse	CAG TGC GGT TCC AGA CAT AG
Gapdh (14433)	Forward	AGG CCG GTG CTG AGT ATG TC	95°C, 8 s; 59°C, 8 s; 72°C, 8 s	332
	Reverse	TGC CTG CTT CAC CAC CTT CT
β-Actin (11461)	Forward	TCT TGG GTA TGG AAT CCT GTG	95°C, 8 s; 60°C, 8 s; 72°C, 8 s	82
	Reverse	AGG TCT TTA CGG ATG TCA ACG