Identification of Urinary Biomarkers for Exercise-Induced Immunosuppression by iTRAQ Proteomics

Figure 2

iTRAQ labeling and basic data analysis. (a) Experimental workflow for iTRAQ labeling and analysis. iTRAQ 8-plex labeling was performed on the collected urine samples. The labeled fractions were combined and subjected to strong cation exchange (SCX) chromatography and desalting, followed by separation using liquid chromatography mass spectrometry (LC-MS/MS), and data analysis and bioinformatics analysis. (b) Mass distribution of all the identified proteins. The horizontal axis is the molecular weight of the identified protein (Unit: kiloDalton, kDa). The vertical axis is the number of proteins identified. (c) The isoelectric point map of all the identified proteins. The horizontal axis is the isoelectric point of the identified protein and the vertical axis is the number of proteins identified. (d) Peptide length distribution of all the identified proteins. The graph shows the percentage of peptides of different lengths in all peptides. The abscissa is the number of peptide amino acid residues, and the ordinate is the number of peptides of this length.

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