Evaluation of Dental Pulp Stem Cell Heterogeneity and Behaviour in 3D Type I Collagen Gels
(a) Representative gelatin zymography gel images of pro- and active MMP-9/MMP-2 activities for high proliferative/multipotent DPSC subpopulation, A3, at early (nonsenescent, 30 PDs) and late (approaching senescence, 80 PDs) stages in their proliferative lifespans, and low proliferative/unipotent DPSCs, A1 (approaching senescence, 17 PDs), following seeding in detached type I collagen gels over 12 days in culture. Pro-MMP-2, active MMP-2, and pro-MMP-9 were detectable at 72 kDa, 62 kDa, and 92 kDa, respectively. Densitometry quantification of (b) pro-MMP-2 and (c) pro-MMP-9 activities for high proliferative/multipotent DPSC subpopulation, A3 (30 PDs and 80 PDs), and low proliferative/unipotent DPSC subpopulation (A1, 17 PDs), following seeding in detached type I collagen gels over 12 days in culture. ,,,.
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