Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway

Table 1

Characteristics of the primers used for real-time PCR.

Genes	Primers (forward and reverse)	Products (bp)
MMP-1	5-CTGCTTACGAATTTGCCGACAGA-3	130
	5-GTTCTAGGGAAGCCAAAGGAGCTG-3
MMP-2	5-GTTCATTTGGCGGACTGTGACG-3	146
	5-ATTCATTCCCTGCAAAGAACACAGC-3
MMP-3	5-GGACAAAGGATACAACAGGGACCA-3	140
	5-GAACCGAGTCAGGTCTGTGAGTG-3
MMP-7	5-GATGGGCCAGGAAACACGC-3	107
	5-CCTAGACTGCTACCATCCGTCCA-3
MMP-9	5-CACGACGTCTTCCAGTACCGAGA-3	115
	5-CATAGGTCACGTAGCCCACTTGGT-3
MMP-10	5-TCTGTTCCTTCGGGATCTGAGATG-3	146
	5-TGAAATTCAGGTTCAGGGTTCCAGT-3
MMP-11	5-ACCTGGACTATCGGGGATGA-3	188
	5-GGCTGGCCATATAGGTGTTG-3
MMP-16	5-GACAGGCCAAAACCTCCTCGG-3	149
	5-TTTCTCACTCGCCAAAACCACTG-3
TIMP-1	5-AAGGCTCTGAAAAGGGCTTC-3	161
	5-GAAAGATGGGAGTGGGAACA-3
TIMP-2	5-TTGACCCAGAGTGGAACG-3	101
	5-ACCAAAGACGGGAGACGA-3
TIMP-3	5-CCTGCTACTACCTGCCTTGC-3	182
	5-TGTGGCATTGATGATGCTTT-3
TIMP-4	5-TCTGGACAGACTGGCTGTTG-3	176
	5-TTGAAGGGATGTGATGGTCA-3
NF-κB p65	5-CAAGTGGCCATTGTGTTCCG-3	149
	5-TGGCGATCATCTGTGTCTGG-3
GAPDH	5-TCGACAGTCAGCCGCATCTTCTTT-3	148
	5-GCCCAATACGACCAAATCCGTTGA-3

MMP: matrix metalloproteinase; TIMP: tissue inhibitors of metalloproteinase.