BioMed Research International / 2020 / Article / Fig 2

Research Article

Arctigenin Inhibits Glioblastoma Proliferation through the AKT/mTOR Pathway and Induces Autophagy

Figure 2

ARG inhibited the migration and invasion of U87MG and T98G in a dose-dependent manner. (a, b) The cells (U87MG and T98G) were incubated in a standard 6-well plate for scratch experiments and were treated with different concentrations of ARG, and pictures were taken under the microscope at 24 h and 48 h. (c, d) Statistical analysis of scratches. (e) U87MG was incubated in the upper chamber of transwell, and different concentrations of ARG (100 μM, 200 μM, and 400 μM) were mixed in the lower chamber of transwell. These cells were stained with crystal violet for at least 30 min, and images were captured with a microscope. (f) Randomly counting the number of cells in the chamber and performing statistical analysis. (g) U87MG was treated with multiple concentrations of ARG for 48 h, and the protein levels of MMP2 and MMP9 were detected by Western blotting, and β-actin was used as a housekeeping protein. Data was expressed as the . vs. the control group. All experiments were repeated three times.
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