Wubeizi Ointment Suppresses Keloid Formation through Modulation of the mTOR Pathway

Figure 5

The effect of Wubeizi ointment on the expression of PI3K, PTEN, Akt, and mTOR. (a) The inhibitory effect of Wubeizi ointment on the mRNA levels of PIK3CA, PTEN, Akt1, and mTOR in keloid fibroblasts. After IGF-1 and/or Wubeizi ointment treatment, the mRNA levels of PIK3CA, PTEN, Akt1, and mTOR in keloid fibroblasts were measured by qPCR. The grey values were quantified with the gel imaging analysis system. The RQ of mRNA were calculated and presented as . ; , . (b) The inhibitory effect of Wubeizi ointment on the protein levels of p-PI3K, PTEN, p-Akt, and p-mTOR in keloid fibroblasts. After IGF-1 and/or Wubeizi ointment treatment, the protein levels of p-PI3K, PTEN, p-Akt, and p-mTOR in keloid fibroblasts were determined by western blot. Representative pictures are shown. Band intensities were quantified with the Image Pro-Plus image analysis system. The relative ratios of proteins to actin band density were presented as . ; , .

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