A Simple Method for the Design and Development of Flavivirus NS1 Recombinant Proteins Using an In Silico Approach
Table 5
The reclassification of each STs by the locus of major epitopes.
DENV1
DENV2
DENV3
DENV4
JEV
WNV
YFV
ZIKV
Major epitope locusa
E1 (28–46)
E1 (14–43)
E1 (28–46)
E1 (14–43)
E1 (14–29)
E1 (14–29)
E1 (19–29)
E1 (14–42)
E2 (96–109)
E2 (161–171)
E2 (161–171)
E2 (96–105)
E2 (96–105)
E2 (64–93)
E2 (70–84)
E3 (159–171)
E3 (197–218)
E3 (197–205)
E3 (143–168)
E3 (146–171)
E3 (147–183)
E3 (147–172)
E4 (204–219)
E4 (232–243)
E4 (209–218)
E4 (197–206)
E4 (193–205)
E4 (211–236)
E4 (197–205)
E5 (317–335)
E5 (268–281)
E5 (317–331)
E6 (317–346)
STb
113
94
64
45
59
75
30
43
EpTc
31
33
9
5
29
29
12
5
Frequency of major EpT (allelic profile) (%)d
EpT 1 (1-1-1-1) (73.9%)
EpT 1 (1-1-1-1-1) (31.5%)
EpT 1 (1-1-1-1) (84.3%)
EpT 1(1) (67.7%)
EpT 1 (1-1-1-1-1-1) (37.9%)
EpT 1 (1-1-1-1-1) (78.6%)
EpT 1 (1-1-1-1) (23.3%)
EpT 1 (1-1-1-1) (88.4%)
EpT 2 (1-6-5-1) (10.1%)
EpT 2 (1-1-3-1-1) (38.2%)
EpT 2 (1-1-1-1) (13.3%)
EpT 1(2) (24.4%)
EpT 1 (1-1-2-3-1-1) (30.7%)
EpT 2 (1-4-1-1) (20.6%)
EpT 3 (1-1-1-2) (5.9%)
aMajor epitope locus is the region in which the core peptides (Tables 1–4) are distributed. The number in parentheses is the amino acid sequence number. bSequence type (ST) was classified by the phylogenetic analysis. The numbers in this row represent the total sum of each ST. cAll the variations of epitopes were assigned allele numbers and combined into an allelic profile and assigned an epitope type (EpT). The numbers in this row represent the total sum of each EpT. dThe frequency registered on GenBank.
