| (a) |
| (b) |
| (c) |
| (d) |
| (e) |
| (f) |
Research Article
Beneficial Effects of Crocin against Depression via Pituitary Adenylate Cyclase-Activating Polypeptide
Figure 4
Crocin relieved corticosterone induced PC12 injury by regulating PACAP-mediated ERK and CREB signaling pathways. (a) Hoechst 33258 staining and observation of PC12 cells with fluorescence microscope. (b) Effect of crocin in cell viability using MTT assay. ANOVA, , . (c-f) PACAP, ERK, and CREB were analyzed by immunoblotting. ANOVA, , ; ANOVA, , ; ANOVA, , . Data are expressed as (). and versus the control group; and versus CORT group.