Dysregulated Bone Metabolism Is Related to High Expression of miR-151a-3p in Severe Adolescent Idiopathic Scoliosis

Figure 3

Expression levels of miR-151a-3p and GREM1, a predicted target gene of miR-151a-3p, in spinal bone tissues from severe scoliosis patients. (a) Total RNA was extracted from the cancellous bone of the facet joint harvested from severe AIS patients undergoing corrective surgery and nonscoliosis patients undergoing spine surgery. Relative miRNA expression levels of miR-151a-3p were determined by quantitative real-time PCR. (b) Venn diagram analysis of target genes predicted by TargerScan7.2 and miRDB. (c) Relative mRNA expression levels of GREM1, ME1, PFN2, YTHDF3, and NAMPT from total RNA extracted from the cancellous bone of severe AIS and nonscoliosis patients. (d, e) GREM1 protein expression in primary osteoblasts extracted from severe AIS and nonscoliosis patients was analyzed by Western blotting. GAPDH was used as an internal reference. Gray value quantitative analysis was performed and reported. The mean value of the nonscoliosis group was set to 1.0. Data are displayed as the . versus the NC group ().

(a)

(b)

(c)

(d)

(e)