High Glucose Induces the Loss of Retinal Pericytes Partly via NLRP3-Caspase-1-GSDMD-Mediated Pyroptosis

<div>High glucose induced inflammation, pore formation, and cell death. HRPs were firstly treated with 30 mM glucose for 12, 24, and 48 h or were treated with the indicated concentrations of high glucose (HG) or mannitol (osmotic pressure control, OP) for 48 h. (a) Cell viability was determined by MTT analysis. (b) IL-1<i>β</i> and IL-18 levels in the cell culture supernatant were determined by ELISA. (c) Fluorometric plots show PI uptake (RFU (%)) over time to reveal the kinetics of pore formation. (d) Cell death was monitored by measuring LDH released (LDH release (%)) by dying cells. 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BioMed Research International

fig1

Figure 1

Figure 1: High Glucose Induces the Loss of Retinal Pericytes Partly via NLRP3-Caspase-1-GSDMD-Mediated Pyroptosis 