High Glucose Induces the Loss of Retinal Pericytes Partly via NLRP3-Caspase-1-GSDMD-Mediated Pyroptosis

<div>shRNA-GSDMD in part reversed the high glucose-induced inflammation and pyroptosis. (a) Western blotting detected the protein expression of GSDMD and GSDMD-N in HRPs treated with shRNA-GSDM seq1 or shRNA-GSDM seq2. 200 <i>μ</i>M glyburide or 10 <i>μ</i>M YVAD was used as intervention reagents; 30 mM high glucose- (HG-) induced (b) NLRP3, cleaved caspase-1, cleaved IL-1<i>β</i>, and GSDMD-N expression, (c) IL-1<i>β</i> and IL-18 release, (d) pore formation, and (e) LDH release were detected by western blotting, ELISA, PI staining, or LDH-release kit, respectively. 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BioMed Research International

fig4

Figure 4

Figure 4: High Glucose Induces the Loss of Retinal Pericytes Partly via NLRP3-Caspase-1-GSDMD-Mediated Pyroptosis 