Association between APOBEC3H-Mediated Demethylation and Immune Landscape in Head and Neck Squamous Carcinoma

Figure 6

Correlations among APOBEC3H, CXCL10, and CD8+ T cell infiltration in HNSC. (a) The differentially methylated CpG sites of CXCL10 in the APOBEC3H-high and APOBEC3H-low groups. (b) The mRNA levels of CXCL10 in the APOBEC3H-high and APOBEC3H-low groups. (c–g) Spearman analysis of the correlations between CXCL10 methylation levels and APOBEC3H mRNA levels (c), CXCL10 mRNA levels and methylation levels (d), APOBEC3H and CXCL10 mRNA levels (e), CXCL10 mRNA levels and CD8+ T cell infiltration (f), and APOBEC3H mRNA levels and CD8+ T cell infiltration (g) in HNSC tumors. Data were analyzed using the TIMER and the CIBERSORT dataset. (h) Kaplan-Meier analysis was employed to determine the OS according to the CD8+ T cell infiltration (high vs. low). Data were analyzed using the TIMER dataset. (i) The qPCR results of APOBEC3H mRNA levels in normal cell line (HOK), dysplasia cell line (DOK), and HNSC cell lines (SCC1, UM1, HSC3, HSC6, CAL27, CAL33, and HN6). (j, k) The overexpression plasmids (APOBEC3H and Vector) were transiently transfected in HSC3 and HSC6 cells. The qPCR analysis was performed to confirm (j) the transfection efficiencies of APOBEC3H and (k) the mRNA levels of CXCL10. . . Student’s -tests.

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