Research Article
Preclinical Development of Mahanine-Enriched Fraction from Indian Spice Murraya koenigii for the Management of Cancer: Efficacy, Temperature/pH stability, Pharmacokinetics, Acute and Chronic Toxicity (14-180 Days) Studies
Figure 2
MEFM2 induced apoptosis in cancer cells and reduced tumor mass in syngeneic cancer mouse models. (a) Ovarian cancer cell lines were treated with MEFM2 at different concentrations and stained with DAPI. The images were captured under a florescence microscope. Arrows indicate the condensed and fragmented nucleus. (b) MEFM2-treated ovarian cancer cells were stained with annexin V/PI and analyzed by FACS. (c) A representative image of the ID8 tumors from the control and the treated mouse. (d) Graph showing the tumor size variation between MEFM2 treated (300 mg/kg BW/day) and vehicle control in ovarian cancer-bearing Balb/c mice. (a–d) Similar experiments were previously done with pure mahanine [10]; however, here we repeated the experiment with MEFM2 for comparison. (e) A representative image of the 4T1 tumors from the control and the treated mouse. (f) Graph showing the tumor size variation between MEFM2 treated (300 mg/kg BW/day), vehicle control, and positive control (paclitaxel) in breast cancer-bearing Balb/c mice. Error bars in all graphs represent the . There is a significant difference () between control and treated groups in their tumor sizes and body weights.
| (a) |
| (b) |
| (c) |
| (d) |
| (e) |
| (f) |