Preclinical Development of Mahanine-Enriched Fraction from Indian Spice Murraya koenigii for the Management of Cancer: Efficacy, Temperature/pH stability, Pharmacokinetics, Acute and Chronic Toxicity (14-180 Days) Studies

Table 1

Antiproliferative activity of MEF_M2 in ovarian and breast cancer cell lines.

Cancer type	Cell lines	Mutation/drug resistant	Culturing media + 10% FBS	MEFM2IC50 (μg/mL)
Ovarian (human)	PA1	NRAS mutation, anoikis resistant	MEM
	OVCAR3	TP53 mutation, drug resistant	IMDM
Ovarian (mouse)	ID8	TP53, BRCA1, and BRCA2 wild type	RPMI-1640
Breast (human)	MCF7	ESR1 mutant	MEM
Breast (mouse)	4T1	P53 null mutant	RPMI-1640

In vitro activity of MEF_M2 in ovarian and breast cancer cell lines was presented. This data was derived from three individual experiments and the was indicated for IC50 values. Anti-proliferative activity of MEF [14] and its biomarker, mahanine [10], in PA1 and OVCAR3 cell lines were previously reported by our group, however we have repeated here with MEF_M2 for comparison.