The PARK2 Mutation Associated with Parkinson’s Disease Enhances the Vulnerability of Peripheral Blood Lymphocytes to Paraquat

Figure 1

Lymphocytes in PD patients are more sensitive to mitochondrial damage and apoptosis induced by paraquat. (a) After treatment with 1 mM paraquat, the ROS levels of immortalized lymphocytes were detected by stained with DCFH-DA (green). The nuclei were counter-stained with Hoechst 33342 (blue). (b) Quantification of DCFH-DA. (c) Quantification of the activity of mitochondrial complex I of immortalized lymphocytes from controls, sporadic PD patients, and PD patients with Parkin mutantion, following treatment with DMSO (control) or paraquat. (d) Quantification of apoptosis of immortalized lymphocytes from controls, sporadic PD patients, and PD patients with Parkin mutation, following treatment with DMSO or paraquat. , ; ; .

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