Peptidomics Analysis Discloses That Novel Bioactive Peptides Participate in Necrotizing Enterocolitis in a Rat Model

Table 1

Differential peptides in the intestine tissue of NEC rat model.

Accession	Gene	Peptide	Fold change	value
Upregulated peptides
G3V8B3	Hist1h2bq	AVTKYTSSK	57.72612	<0.05
P69897	Tubb5	FVFGQSGAGNN	31.84287	<0.01
Q10758	Krt8	DGKLVSESSDIMSK	25.55914	<0.001
Q10758	Krt8	LVSESSDIMSK	22.05736	<0.001
P15999	Atp5a1	VGLKAPGIIPR	21.49717	<0.001
O88989	Mdh1	VIVVGNPANTNCLTASK	12.14385	<0.05
Q66HT1	Aldob	ALQASALAAWGGK	11.93121	<0.001
G3V8B3	Hist1h2bq	LLLPGELAKH	11.86002	<0.001
P04182	Oat	ALQDPNVAAF	11.16052	<0.05
P05197	Eef2	VFSGVVSTGLKVR	10.57569	<0.05
Q10758	Krt8	QPGFGSVGGSST	10.57101	<0.01
F1LNF1	Hnrnpa2b1	GFGFVTF	10.55872	<0.05
K7S2S2	Hist2h2aa3	AGLQFPVGR	10.3368	<0.05
Q7M0E3	Dstn	FLWAPEQAPLKSK	10.01604	<0.01
Q10758	Krt8	GGLTSPGFS	9.126557	<0.001
M0R7B4	Hist1h1d	SGVSLAALKK	8.945185	<0.001
Q10758	Krt8	FQPGFGSVGGSST	8.793551	<0.001
P69897	Tubb5	FVFGQSGAGN	8.771316	<0.05
D3ZGY4	Gapdh-ps2	GAAQNIIPASTGAAKAVGK	8.399639	<0.001
D3ZBN0	Hist1h1b	SLVSKGTLVQTK	8.352781	<0.01
M0R7B4	Hist1h1d	GILVQTKGTGASGS	8.334644	<0.001
P02262	NA	VGAGAPVYL	8.301511	<0.001
Q10758	Krt8	GMSSFQPGFGSVGGSST	8.18755	<0.01
P10719	Atp5b	IGLFGGAGVGK	7.935978	<0.01
B5DEM5	Rpl14	AAIAAAAAAK	7.751116	<0.01
K7S2S2	Hist2h2aa3	GLQFPVGR	7.629358	<0.05
P62630	Eef1a1	ESFSDYPPLGR	7.607978	<0.001
O55159	Epcam	QKDCVCNNYKLTSR	7.485879	<0.001
P15865	Hist1h1e	SETAPAAPAAPAPAEKTPIKKK	7.38236	<0.01
D3ZGY4	Gapdh-ps2	VKVGVNGFGR	7.332894	<0.001
F1M577	LOC100359671	AFIAHPKLGK	6.954795	<0.05
Q6P725	Des	TFGGAPGFSLGSPLS	6.720618	<0.05
G3V8B3	Hist1h2bq	LLLPGELAK	6.595403	<0.01
D3ZIE9	Aldh18a1	VGLGGMEAKVK	6.321181	<0.001
Q925G0	Rbm3	DYSGSQGGYDRYSGGN	6.259407	<0.01
Q10758	Krt8	SFQPGFGSVGGSST	6.237045	<0.01
Q10758	Krt8	SSFQPGFGSVGGSST	6.119025	<0.01
Q9QXQ0	Actn4	STALYGESDL	5.964186	<0.001
Q10758	Krt8	GSLGGFGGAGVGGIT	5.600793	<0.05
P05065	Aldoa	ALQASALK	5.474993	<0.01
M0RA08	Plin3	VTGAVDVTCGAVK	5.466526	<0.001
Q6P9V9	Tuba1b	VAEITNACFEPAN	5.315759	<0.05
A0A0G2K3Z9	NA	SSGNAKIGHPAPSFK	4.969645	<0.01
Q66H80	Arcn1	VLLAAAVCTK	4.865557	<0.05
D3ZGY4	Gapdh-ps2	ALNDNFVK	4.838244	<0.05
B0K031	Rpl7	IALTDNSLVAR	4.834916	<0.001
P62268	Rps23	KANPFGGASHAK	4.801351	<0.001
D3Z7Y6	Krt20	LGVAPSVYGGAGGHGT	4.677876	<0.001
Q10758	Krt8	KLEVDPNIQAVR	4.663074	<0.001
D3ZBN0	Hist1h1b	SLVSKGTLVQTKGTGASGSF	4.551461	<0.01
M0R7B4	Hist1h1d	SGVSLAALK	4.443164	<0.01
P05197	Eef2	VNFTVDQIR	4.417121	<0.01
V5QR27	NA	FPGQPGGPGA	4.341792	<0.05
P63029	Tpt1	LDYREDGVTPF	4.230072	<0.05
Q5EB49	Eno1	SFRNPLAK	4.118444	<0.05
Q80ZE7	Cd2ap	GLPAGGIQPHPQTK	3.871932	<0.01
M0R7B4	Hist1h1d	ASGPPVSELITK	3.803902	<0.001
P18420	Psma1	LVSLIGSK	3.745425	<0.05
P13437	Acaa2	GVFIVAAK	3.727173	<0.01
D3ZGY4	Gapdh-ps2	GAAQNIIPASTGAAK	3.718082	<0.001
Q10758	Krt8	SLGGFGGAGVGGIT	3.670916	<0.001
D3ZBN0	Hist1h1b	ALAAGGYDVEKNNSR	3.654154	<0.05
Q9WVK7	Hadh	TGEGFYKYK	3.578854	<0.01
K7S2S2	Hist2h2aa3	AGLQFPVGRVH	3.386712	<0.05
A0A0G2K6S9	Myh11	STVAALEAK	3.278155	<0.01
P06302	Ptma	SDAAVDTSSEITTK	3.185452	<0.05
G3V779	Lad1	TEVLVTPAGVASK	3.18017	<0.01
B2GVB1	S100a6	LIYNEALK	3.138931	<0.001
K7S2S2	Hist2h2aa3	AEILELAGNAAR	3.121051	<0.01
P69897	Tubb5	YNEATGGKYVPR	3.110143	<0.05
D3Z7Y6	Krt20	SLSSSSQGPALSTSGSL	3.061158	<0.05
P63018	Hspa8	SKGPAVGIDLGTT	3.057092	<0.05
A0A0G2JWK7	Tagln	EFTDSQLQEGK	3.027585	<0.05
P62630	Eef1a1	QTVAVGVIK	3.020094	<0.05
P21913	Sdhb	AQTAAAAAPRIK	2.929567	<0.01
Q5RJK6	Inpp1	LIQSLGPLKT	2.899027	<0.01
P10860	Glud1	VVQGFGNVGLH	2.760697	<0.05
P47875	Csrp1	GFGFGQGAGALVH	2.639933	<0.001
Q63191	Mamdc4	ADQVTLPESITSNP	2.631403	<0.01
Q07936	Anxa2	STVHEILCKL	2.621438	<0.001
P06302	Ptma	AAVDTSSEITTK	2.568546	<0.01
Q3MHS9	Cct6a	AAVKTLNPKAEVAR	2.554117	<0.05
P38552	Lgals4	YPSAGYNPPQ	2.508311	<0.05
P05197	Eef2	VAVEAKNPADLPK	2.48379	<0.01
Q5RKI0	Wdr1	SVADGYSENNVF	2.474195	<0.001
G3V8B3	Hist1h2bq	HAVSEGTKAVTKYTSSK	2.468693	<0.05
Q6URK4	Hnrnpa3	YGGGGNYNDFGN	2.394012	<0.05
Q6VPP3	Clca4	DTQPVLENFSR	2.382552	<0.05
P12346	Tf	LLEACTFHKS	2.333879	<0.05
P63029	Tpt1	GAIDDSLIGGN	2.312302	<0.001
Q10758	Krt8	GGFGGAGVGGIT	2.276436	<0.05
Q07936	Anxa2	STVHEILCK	2.247658	<0.01
P00731	Cpa1	ALSTDSFNYATYH	2.173098	<0.05
Q6URK4	Hnrnpa3	SGSPYGGGYGSGGGSGGYGS	2.128543	<0.05
P38552	Lgals4	YPSAGYNPPQMN	2.11776	<0.001
Q4FZY0	Efhd2	ATDELASKLSR	2.05165	<0.05
A0A0G2QC04	Pls1	EGITAIGGTSSI	2.004625	<0.01
P38552	Lgals4	PAYPSAGYNPPQMN	1.905471	<0.001
Q9Z144	Lgals2	SEKFEVTNLNMK	1.899939	<0.05
D3ZVN3	Fbxo10	HNAEAGVDIR	1.77112	<0.05
G3V779	Lad1	QQVVGAVQAPGQEKVE	1.722176	<0.01
P46462	Vcp	AVANETGAF	1.668905	<0.001
D4A3Z8	Tmco3	LRIRPTQSV	1.649045	<0.05
Downregulated peptides
A0A0G2K890	Ezr	FVIKPIDKK	-1.50403	<0.05
Q9JJ19	Slc9a3r1	EALVEPASESPRPA	-1.52587	<0.05
K7S2S2	Hist2h2aa3	AQGGVLPNIQAV	-1.53578	<0.05
P15865	Hist1h1e	APAAPAAPAPAEKTP	-1.57197	<0.001
F1M4J0	Rictor	STELLLGV	-1.57785	<0.05
A0A0G2JUA5	Ahnak	DVDVQGPDWH	-1.61943	<0.01
Q63191	Mamdc4	NADQVTLPESITSNP	-1.64595	<0.05
Q6P9V9	Tuba1b	DLEPTVIDEVR	-1.66594	<0.05
B5DFA0	Vil1	EENQVITPRLF	-1.69645	<0.05
A0A0G2K3K2	Actb	NELRVAPEEHPV	-1.70784	<0.05
Q63191	Mamdc4	QVTLPESITSNP	-1.7079	<0.05
D3ZDZ1	Apbb1ip	LPPPPPPP	-1.72276	<0.01
Q5EB49	Eno1	KLNVVEQEKIDQLMIEMDGTENK	-1.7416	<0.05
P62630	Eef1a1	GVGEFEAGISKN	-1.78783	<0.01
P63269	Actg2	FAGDDAPRAVFPS	-1.81415	<0.001
D3ZBN0	Hist1h1b	GTGASGSFKLN	-1.85091	<0.001
D3ZAU0	Muc5b	ITFCEGSCSGK	-1.90446	<0.05
P06302	Ptma	SDAAVDTSSEITTKDL	-1.93122	<0.05
D3ZX87	LOC100910017	KNLQTVNVDEN	-1.93203	<0.05
D3ZUM4	Glb1	LELCTVEFVDTPVIG	-1.97941	<0.01
Q6P9V9	Tuba1b	QPPTVVPGGDLAK	-2.01585	<0.05
P63312	Tmsb10	ADKPDMGEIASFD	-2.02372	<0.001
D4A269	NA	ADEIAKAQVA	-2.02826	<0.001
A0A0G2K890	Ezr	APPPPPPPVYEPVN	-2.10935	<0.01
F1LQ48	Hnrnpl	DEGYGPPPPHYE	-2.13291	<0.01
P08081	Clta	APAGAPGGPALGN	-2.17074	<0.001
P06761	Hspa5	MKETAEAYLGKK	-2.21256	<0.01
Q62803	Spam1	LEDDLVNTIGEIV	-2.21478	<0.05
P38552	Lgals4	IPAYPSAGYNPPQ	-2.21577	<0.01
A0A0G2K6S9	Myh11	AQKGQLSDDEKF	-2.22383	<0.05
P13437	Acaa2	SACIGGGQGISLIIQNTA	-2.23257	<0.01
P30009	Marcks	SPEAPPAPVAE	-2.24701	<0.001
P45592	Cfl1	ASGVAVSDGVIK	-2.24734	<0.001
O55212	NA	DAGAAGGPGGPGGPGLG	-2.30987	<0.05
D4AE06	Fkbp15	TPSVQPSLQPSHP	-2.32989	<0.05
P62630	Eef1a1	SGDAAIVDMVPGKP	-2.36181	<0.001
P63269	Actg2	FAGDDAPRAVFPSIV	-2.37939	<0.05
P62630	Eef1a1	FAPVNVTTEVK	-2.45167	<0.01
P30009	Marcks	CSPEAPPAPVAE	-2.54825	<0.01
A0A0G2K3K2	Actb	DSGDGVTHTVPIYE	-2.55819	<0.01
D3ZFU9	Mylk	AQPVGALKPIGN	-2.77191	<0.05
P15865	Hist1h1e	SETAPAAPAAPAPAEKTPIK	-2.77847	<0.001
Q53Z83	Mt1	SCCSCCPVGCSKCAQGCVC	-2.79709	<0.05
D3ZDZ1	Apbb1ip	NFLPPPPPPP	-2.8025	<0.001
P63312	Tmsb10	ADKPDMGEIAS	-2.84338	<0.001
Q6URK4	Hnrnpa3	SPYGGGYGSGGGSGGY	-2.93019	<0.01
Q10758	Krt8	GFGGAGVGGIT	-2.95692	<0.01
P62630	Eef1a1	VGEFEAGISKN	-2.98559	<0.001
D3ZFU9	Mylk	AKPAETLKPVGN	-2.98675	<0.01
A0A0G2K890	Ezr	APPPPPPPVYE	-3.14377	<0.001
Q91W32	Casp1	KEGGAFPGPSGSLK	-3.15783	<0.001
Q53Z83	Mt1	SCCSCCPVGCSKCAQGCVCKG	-3.27655	<0.01
P06302	Ptma	SDAAVDTSSEITTKDLK	-3.51799	<0.001
B6ID08	Mt2A	SCCSCCPVGCAKCSQGCICK	-3.56818	<0.05
Q6P9V9	Tuba1b	LEPTVIDEVR	-3.6423	<0.001
P62630	Eef1a1	AGVGEFEAGISKN	-3.79477	<0.01
D3ZJF8	Fcgbp	CVAESTAVCRAQGD	-3.88626	<0.001
A0A0G2K890	Ezr	AELSSEGILDDRN	-3.89919	<0.05
A0A0G2JUA5	Ahnak	EGPEVDVSLP	-4.05973	<0.01
Q53Z83	Mt1	CAQGCVCKGASDKCTCCA	-4.23021	<0.01
Q63041	A1m	AVDQSVLLLKPEAK	-4.3155	<0.001
D3ZBN0	Hist1h1b	GPPVSEL	-4.64319	<0.001
P13668	Stmn1	ASSDIQVKELE	-4.88393	<0.01
P97881	Muc13	GVPSQTSNPYAN	-5.02829	<0.001
Q63041	A1m	ALVQKDTVVKPVIVEPEG	-5.23966	<0.01
Q6P9V9	Tuba1b	TVVPGGDL	-5.71525	<0.001
P15865	Hist1h1e	SETAPAAPAAPAPAEKTP	-6.05552	<0.001
Q7M0E3	Dstn	FLWAPEQAPLK	-6.43258	<0.01
P62630	Eef1a1	ALPGDNVGFN	-6.78263	<0.001
Q5PQK2	Fus	YPTQPGQGYSQQSN	-8.52323	<0.05
Q10758	Krt8	FGGAGVGGIT	-8.58147	<0.001
P62630	Eef1a1	APVNVTTEVK	-13.4474	<0.001
P63269	Actg2	GQKDSYVGDEAQSKRG	-15.8726	<0.001