Histone Deacetylase Inhibition Attenuates Aortic Remodeling in Rats under Pressure Overload

Figure 4

Effect of MGCD on the expression of aortic remodeling markers in rats after TAC. Immunohistochemistry of the aortic wall from the sham (), sham plus MGCD (), TAC (), and TAC plus MGCD groups () was performed using the anti-αSMA (a), anti-MMP2 (b), and anti-MMP9 (c) antibodies. The TAC rats had higher αSMA, MMP2, and MMP9 expression levels (brown stain) than the sham rats, but treatment with MGCD decreased their expression in the TAC rats. Scale bars are 50 μm. mRNA expression of αSMA (d), Mmp2 (e), and Mmp9 (f), markers for myofibroblasts and aortic remodeling, was determined by qRT-PCR. The TAC rats exhibited increased αSMA, Mmp2, and Mmp9 mRNA expression, but these decreased with MGCD administration. Data are shown as of eight rats per group ( and vs. sham group, ^# vs. TAC group).