Research Article
miR-21-5p Suppresses Mitophagy to Alleviate Hyperoxia-Induced Acute Lung Injury by Directly Targeting PGAM5
Figure 5
miR-21-5p directly targets PGAM5 to regulate mitophagy in AEC-II cells. (a) Sequence alignment of the PGAM5 3UTR with miR-21-5p. (b) The activity of luciferase reporters containing wild-type (WT) or mutant (MUT) PGAM5 3UTR that were treated with miR-21-5p mimics or mimic control (mimic-NC). (c) The transfection efficiency of miR-21-5p mimic and PGAM5 plasmid in AEC-II cells. (d) Representative confocal microscopy images of JC-1 staining and (e) MitoTracker Red/GFP-LC3 staining of the AEC-II cells with different treatments were performed. Images showed representative examples from three independent experiments. μm. (f) Representative blots and densitometric analyses were shown for the expression of PGAM5, LC3, PINK1, Parkin, and COX IV in AEC-II cells after different treatments. . Data are presented as the . , , and vs. the control group; #, ##, and ###vs. the PGAM5 OE group.
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