A Novel Imidazopyridine Derivative Exerts Anticancer Activity by Inducing Mitochondrial Pathway-Mediated Apoptosis
Compound 9i induces mitochondria-dependent apoptosis in human cervical cancer cells. (a) Representative scatter plots showing the distribution of rhodamine 123 and PI staining for control- and compound 9i-treated HeLa cells. DMSO was used as a vehicle control. (b) Quantitative analysis of the percentage of cells with high by FACS analysis. Values are represented as the (). Significance was tested by Student’s -test (, versus DMSO-treated cells). (c) Western blot was used to determine the expression level of apoptosis-related proteins in DMSO- or compound 9i-treated HeLa cells. The intensity of the bands was measured, and the fold change of the intensity was compared with that of the control and indicated below the bands. The expression of Bcl-xL was statistically analyzed in Figure S2b. Tubulin was used as a loading control. The results were representative of three independent experiments.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.