Research Article

Polyphyllin I Promoted Melanoma Cells Autophagy and Apoptosis via PI3K/Akt/mTOR Signaling Pathway

Figure 6

Polyphyllin I inhibited PI3K/AKT/mTOR signaling pathways in A375 melanoma cells. (a) Western blot assay was performed to assess the expressions of proteins related with PI3K/AKT/mTOR signaling pathways. (b–d) Polyphyllin I treatment dramatically attenuated the expressions of p-PI3K, p-AKT, and p-mTOR, but made no difference to the protein expressions of PI3K, AKT, and mTOR. IGF-1 treatment alleviated distinctly the effects of Polyphyllin I on the expressions of p-PI3K, p-AKT, and p-mTOR. in comparison with 0 mg/L PPI group; # in comparison with 6 mg/L PPI group. Note: 1: 0 mg/L PPI group; 2: 1.5 mg/L PPI group; 3: 3.0 mg/L PPI group; 4: 6.0 mg/L PPI group; 5: 6.0 mg/L PPI +20 mg/L IGF-1group.
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