The Oncogenic Role of miR-BART19-3p in Epstein-Barr Virus-Associated Diseases

Figure 5

APC is the target of miR-BART19-3p. (a) q-PCR validation of selected genes downregulated in CNE2 cells expressing miR-BART19-3p. (b) Predicted miRNA binding sites within the 3-UTR of APC mRNA. The seed sites and the mutation in seed sites of miR-BART19-3p were shown. (c) Luciferase activity was measured in CNE2 cells transfected with the APC 3-UTR luciferase reporter vector and miR-BART19-3p mimic, miR-BART19-3p-mut, or relative miRNA control. (d) APC mRNA expression levels in EBV-positive and EBV-negative cells after miR-BART19-3p mimic or miR-BART19-3p inhibitor treatment. (e) Expression of APC protein is reduced by the miR-BART19-3p mimic and increased by the miR-BART19-3p inhibitor. (f) EdU incorporation assay was measured after transfection of miR-BART19-3p mimic and siR-APC at 48 h in CNE2. (g) EdU incorporation assay was measured after transfection of miR-BART19-3p inhibitor and siR-APC at 48 h in C666-1. , , Student’s -test, independent experiments.

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