Proteomic Analysis of Human Endometrial Tissues Reveals the Roles of PI3K/AKT/mTOR Pathway and Tumor Angiogenesis Molecules in the Pathogenesis of Endometrial Cancer

Figure 2

The distribution of quantitative proteomics using Gaussian fit and determination of altering proteins. (a) The Venn diagram representing the overlap of quantified proteins in two biological replicates, respectively. (b) Histogram showing the number of peptides matched to proteins. The -axis displays the number of identified peptides. The primary -axis indicates the number of identified proteins (bars). The second -axis represents the percent (lines). (c) Scatter plots of the two biological replicates of Con 1 and Con 2, iTRAQ 116, 117-labeled Exp 1 and Exp 2. (d) Histograms of log₂ ratios of the abundance of qualified proteins between Con 1 and Con 2 (). (e) Histograms of log₂ ratios of the abundance of qualified proteins between Exp 1 and Exp 2 (). (f) Histograms of log₂ ratios of the abundance of qualified proteins between Exp and Con (). (g) The comparison of log₂ ratio distributions of quantified proteins in (a) to (c). (h) Global distribution of the overlap of the qualified proteins in twice independent repeat experiment (). (i) Distribution of 619 differentially expressed proteins in twice independent repeat experiments. Of these, 327 proteins exhibited downregulated expression and 292 proteins exhibited upregulated expression.

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