Minocycline Induces Autophagy and Inhibits Cell Proliferation in LPS-Stimulated THP-1 Cells
Minocycline suppressed cytokine and chemokine production in LPS-stimulated THP-1 cells in dose-dependent and time-dependent manner. (a, b) THP-1 cells were stimulated with LPS (1 μg/ml) alone or LPS and (10-100) μg/ml minocycline for 4 h ( vs. control; ▲,▲▲ vs. LPS). (c, d) THP-1 cells were stimulated with LPS (1 μg/ml) alone or LPS and 50 μg/ml minocycline for 1, 2, 4, or 12 h (;). (e, f) The relative cell viability of THP-1 cells stimulated with minocycline or LPS and minocycline for 24 h ( vs. control). The concentration of TNF-α or IL-8 in the supernatants was measured by ELISA. Data were represented as the (). Mino: minocycline.
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