BioMed Research International / 2020 / Article / Fig 6

Research Article

Minocycline Induces Autophagy and Inhibits Cell Proliferation in LPS-Stimulated THP-1 Cells

Figure 6

The cooperative effect of rapamycin and minocycline inhibited cell proliferation in LPS-stimulated THP-1cells. (a) THP-1 cells were stimulated by LPS with concentration from 0.1 to 10 μg/ml for the indicated time. (b) THP-1 cells were treated with minocycline with the concentration from 0.5 to 50 μg/ml for the indicated time. (c) THP-1 cells were treated with 1 μg/ml LPS and 50 μg/ml minocycline for the indicated time. (d) THP-1 cells were preincubated with 5 μM rapamycin for 1 h, followed with 1 μg/ml LPS and 50 μg/ml minocycline for the indicated time. Cell proliferation was assessed by WST-1 assay and expressed as the percentage of viability compared to control. Data were represented as the (, , ).
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