Research Article

Peptide Extracts from Native Lactic Acid Bacteria Generate Ghost Cells and Spheroplasts upon Interaction with Salmonella enterica, as Promising Food Antimicrobials

Figure 1

The cytoplasmic membrane permeation of Salmonella enterica subsp. enterica ATCC51741. Bacteria (after 120 min incubation) were removed by centrifugation, and enzyme release was assayed in the cell-free supernatant. Legend: 1 X MIC of Gt2, 1 X MIC of Cys5-4, 1 X MIC of Gt28 peptide extracts; Triton X-100 (0.1%); control: untreated cells. Results are representative of three independent experiments each made in triplicate. The release of o-nitrophenol (ONP) per minute per milliliter and calculated as described: , where A415 was the absorbance at 415 nm and 4.86 was the coefficient of extinction (mM-1 cm-1) of ONP, respectively.