Research Article

Functionalized Scaffold and Barrier Membrane with Anti-BMP-2 Monoclonal Antibodies for Alveolar Ridge Preservation in a Canine Model

Figure 5

Histology at low magnification in the (a) anti-bone morphogenetic protein 2 monoclonal antibody- (anti-BMP-2 mAb-) treated site and (b) isotype matched control mAb site. Outline of alveolar bone in control sites appeared as a knife-edged shape due to buccal bone loss. The residual barrier collagen membrane (CM) was observed in both test and control sites (green dotted lines). Osteogenesis was observed within the entire extraction socket of both test and control sites. Histology at high magnification in (c) anti-BMP-2 mAb-treated and (d) isotype matched control mAb sites. The micrographs showed osteoblast-like cells (yellow arrowheads) as well as blood vessels (pink arrowheads) participating in active bone formation. The superficial anorganic bovine bone mineral (ABBM) particles (black asterisks in d) in control sites showed fibrotic encapsulation, while osteogenic cells as well as osteoid bone formation around residual ABBM graft particles were noted in test sites (white asterisk in c).
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