The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1

Localization of Borealin in the midbody depends on IQGAP1. (a). HeLa cells were treated with siRNAs directed against IQGAP1 or control. After 48 h, cell lysates were detected by western blotting with the indicated antibodies. (b). HeLa cells were treated with siRNAs direct against IQGAP1 or control. After 48 h, cells were fixed and stained with Borealin antibody (green), alpha-tubulin antibody (red), and DAPI (blue).  μm. The graph below shows the overlap of fluorescence intensity peaks along profile localization of Borealin on midbody in the merged micrograph. (c) The percentage of Borealin midbody location disturbed in IQGAP1-depleted cells and in control cells. A minimum of 150 cells were counted per sample in three independent experiments. Error bars represent ±S.E.