Research Article

Identification of Recurrent Variants in BRCA1 and BRCA2 across Multiple Cancers in the Chinese Population

Figure 3

(a) The BRCA1 mRNA level of MDA-MB-231 transfected with BRCA1c.3257del plasmids was significantly reduced () compared to those transfected with wild-type BRCA1 plasmids. Western blot analysis showed that the BRCA1 protein level translated by BRCA1c.3257del-overexpressed cells was lower than BRCA1WT in MDA-MB-231 cells. (b) The colony formation efficiency of MDA-MB-231 cells transfected with BRCA1c.3257del was significantly increased (). (c) Cell proliferation of BRCA1c.3257del (red) and BRCA1WT (blue) was measured using the CCK-8 assay. Increased proliferation of BRCA1c.3257del-overexpressing MDA-MB-231 cells was detected in vitro ().