MAGE-Targeted Gold Nanoparticles for Ultrasound Imaging-Guided Phototherapy in Melanoma

Figure 2

Morphological distribution of MAGE-Au-PFH-NPs under the microscope and photothermal properties of NPs in vitro. (a) CLSM image of DiI-stained MAGE-Au-PFH-NPs (scale bar: 5 μm). (b) TEM image of MAGE-Au-PFH-NP distributions in cells (scale bar: 5 μm). Red arrow showed the NP distribution in B16 cells. (c) Plot of temperature change of MAGE-Au-PFH-NP suspension at different power densities of an 808 nm laser (0.12, 0.42, 0.72, and 1.00 W/cm²) as a function of irradiation duration (MAGE-Au-PFH-NP concentration: 25 mg/mL and 100 μL). (d) Plot of temperature change of NS and MAGE-Au-PFH-NP suspension at different levels of concentration (MAGE-Au-PFH-NP concentrations: 3.125, 6.25, and 13.5, 25 mg/mL and 100 μL) exposure to an 808 nm laser (1.00 W/cm²) as a function of irradiation duration. (e) Confocal fluorescence imaging of Calcein-AM and PI costained B16 cells after coincubation with NPs for 12 h followed by different treatments ().

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