Overexpression of bla_OXA-58 Gene Driven by ISAba3 Is Associated with Imipenem Resistance in a Clinical Acinetobacter baumannii Isolate from Vietnam

Table 1

Primers and probes used for PCR amplification and sequencing of antimicrobial resistance genes and related genetic elements.

Primers/probes	Sequence (5→3)	Length (bp)	Tm (°C)	Product (bp)	Ref.
OXA-23-F	CACTAGGAGAAGCCATGAAGC	21	55.0	114	Nguyen et al., 2017
OXA-23-R	CAGCATTACCGAAACCAATACG	22	55.0
OXA-24-F	GCTAAATGCTTTAATCGGGCTAG	24	55.0	141	Nguyen et al., 2017
OXA-24-R	ACTGGAACTGCTGACAATGC	20	55.0
OXA-51-F	GAAGTGAAGCGTGTTGGTTATG	22	55.0	148	Nguyen et al., 2017
OXA-51-R	GCCTCTTGCTGAGGAGTAAT	20	55.0
OXA-51-P	FAM-CGACTTGGGTACCGATATCTGCATTGC-BHQ1	27	61.3		This study
OXA-58-F	ATATTTAAGTGGGATGGAAAGCC	23	55.0	110	Nguyen et al., 2017
OXA-58-R	CGTGCCAATTCTTGATATACAGG	23	55.0
OXA-58-P	FAM-TTTACTTTGGGCGAAGCCATGCAAG-BHQ1	25	60.6		This study
16S-rRNA-F	CCAGTGACAAACTGGAGGAAG	21	55.5	199	This study
16S-rRNA-R	GCTGTGTAGCAACCCTTTGTA	21	55.2
16S-rRNA-P	HEX-ACGTCAAGTCATCATGGCCCTTACG-BHQ1	25	61.5
HRF/ISAba1	CACGAATGCAGAAGTTG	17	56.0	520	Segal et al., 2005
HRR/ISAba1	CGACGAATACTATGACAC	18	56.0
ISAba2A	AATCCGAGATAGAGCGGTTC	20	54.0	1200	Poirel et al., 2006
ISAba2B	TGACACATAACCTAGTGCAC	20	52.1
ISAba3A	CAATCAAATGTCCAACCTGC	20	52.3	200	Poirel et al., 2006
ISAba3C	AGCAATATCTCGTATACCGC	20	51.8
ISAba4A	ATTTGAACCCATCTATTGGC	20	50.6	612	Brown et al., 2007
ISAba4B	ACTCTCATATTTTTTCTTGG	20	45.3
IS18A	CACCCAACTTTCTCAAGATG	20	51.2	925	Poirel et al., 2006
IS18B	ACCAGCCATAACTTCACTCG	20	54.7
1512F/ITS	GTCGTAACAAGGTAGCCGTA	20	54.1	607	Chang et al., 2005
6R/ITS	GGGTTYCCCCRTTCRGAAAT	20	56.5
NDM-F	GACCGCCCAGATCCTCAA	18	55.4	52	Yong et al., 2009; CDC 2011
NDM-R	CGCGACCGGCAGGTT	15	57.0
NDM-P	HEX-TGGATCAAGCAGGAGAT-ZEN/IBFQ	17	48.3
KPC-F	GGCCGCCGTGCAATAC	16	56.0	61	Garcia et al., 2010; CDC 2011
KPC-R	GCCGCCCAACTCCTTCA	17	56.5
KPC-P	6FAM-TGATAACGCCGCCGCCAATTTGT-ZEN/IBFQ	23	62.2