miR-30e-3p Promotes Cardiomyocyte Autophagy and Inhibits Apoptosis via Regulating Egr-1 during Ischemia/Hypoxia
Autophagic vacuoles and autophagic flux determined by TEM and confocal microscopy. (a) Autophagic vacuoles measured using TEM in the five groups. Red arrows represent autophagolysosomes or autophagosomes. Visible intracellular double-membrane autophagic vacuoles and mitochondrial swelling after IH exposure, magnification 30,000x. (b) Autophagic flux determined by confocal microscopy with double fluorescence of mRFP-GFP-LC3. Autophagic flux increased significantly at 3 h post IH exposure and then gradually decreased over time. Magnification 400x (; and , compared to the 0 h group). h: hour.
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