BioMed Research International / 2020 / Article / Fig 2

Research Article

miR-30e-3p Promotes Cardiomyocyte Autophagy and Inhibits Apoptosis via Regulating Egr-1 during Ischemia/Hypoxia

Figure 2

Autophagic vacuoles and autophagic flux determined by TEM and confocal microscopy. (a) Autophagic vacuoles measured using TEM in the five groups. Red arrows represent autophagolysosomes or autophagosomes. Visible intracellular double-membrane autophagic vacuoles and mitochondrial swelling after IH exposure, magnification 30,000x. (b) Autophagic flux determined by confocal microscopy with double fluorescence of mRFP-GFP-LC3. Autophagic flux increased significantly at 3 h post IH exposure and then gradually decreased over time. Magnification 400x (; and , compared to the 0 h group). h: hour.
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