miR-30e-3p Promotes Cardiomyocyte Autophagy and Inhibits Apoptosis via Regulating Egr-1 during Ischemia/Hypoxia
Overexpression of miR-30e-3p augments autophagosomes and autophagic flux. (a) Overexpression of miR-30e-3p promotes autophagy. Autophagic vacuoles measured using TEM; red arrows represent autophagolysosomes or autophagosomes. Increase in the number of autophagic vacuoles after miR-30e-3p overexpression, magnification 30,000x. (b) Autophagic flux determined by confocal microscopy. Overexpression of miR-30e-3p increased autophagy. 3-MA inhibited autophagy in miR-30e-3p overexpressing IH-exposed cardiomyocytes, magnification 400x (;, compared to the control group; △△, compared to the IH group; ▽▽, compared to the IH + MIR group). Con: control; NC: negative control; IH: ischemia/hypoxia; MIR: miR-30e-3p; 3-MA: 3-methyladenine.
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