Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer

Figure 5

Transwell invasion assay results expressed as the number of invaded cells per field. cir-ITCH inhibited the invasion abilities of LNCaP cells (a) and PC-3 cells (b) compared with GFP controls, while miR-17 promoted the invasion abilities and counteracted the effect of cir-ITCH. No significant difference was observed in the context of cotransfection (cir-ITCH + miR-17) and the GFP control (magnification, 100x, ; ns: no significance, Student’s -test). Wound-healing assay results were expressed as migration rates. The results showed that overexpression of cir-ITCH resulted in the slower closure of scratch wounds in both LNCaP cells (c) and PC-3 cells (d), while transfection of miR-17 resulted in faster closure. Cotransfection with miR-17 and cir-ITCH resulted in an effect comparable to the GFP control condition (; ns: no significance, Student’s -test).

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