Comparison of Primer-Probe Sets among Different Master Mixes for Laboratory Screening of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Table 1

Volumes of reagents for reactions using two different polymerase enzymes combined with three primer-probe sets.

No.	Reagent	LightCycler Multiplex RNA Virus Master (TIB-Molbiol)	LightCycler Multiplex RNA Virus Master (IDT/Phu Sa)	Invitrogen™ SuperScript™ III One-Step RT-PCR System (TIB-Molbiol)	Invitrogen™ SuperScript™ III One-Step RT-PCR System (IDT/Phu Sa)
1	H2O (RNAse free)	10.4 μL	8.4 μL	5.6 μL	3.6 μL
2	Reaction mix	4.0 μL	4.0 μL	12.5 μL	12.5 μL
3	Primer E_Sarbeco_F1	0.5 μL	1.0 μL	0.5 μL	1.0 μL
4	Primer E_Sarbeco_R2		1.0 μL		1.0 μL
5	Primer E_Sarbeco_R2		0.5 μL		0.5 μL
6	MgSO4 (50 nM)	0.4 μL	-	0.4 μL	0.4 μL
7	RT enzyme	0.1 μL	0.1 μL	1.0 μL	1.0 μL
8	Template RNA	5.0 μL	5.0 μL	5.0 μL	5.0 μL
	Total	20 μL	20 μL	25 μL	25 μL