Comparison of Primer-Probe Sets among Different Master Mixes for Laboratory Screening of Severe Acute Respiratory Syndrome Coronavirus 2 (<i>SARS-CoV-2</i>)

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr class="thead"><td class="align_left">Parameter</td><td class="align_center">RT step</td><td class="align_center">Denaturation</td><td class="align_center" colspan="2">Cycling</td></tr><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr><td class="align_left">Cycles</td><td class="align_center">1</td><td class="align_center">1</td><td class="align_center" colspan="2">45</td></tr><tr><td class="align_left">Target temperature (°C)</td><td class="align_center">55</td><td class="align_center">94</td><td class="align_center">94</td><td class="align_center">58</td></tr><tr><td class="align_left">Duration (hours/minutes/seconds)</td><td class="align_center">00 : 10 : 00</td><td class="align_center">00 : 03 : 00</td><td class="align_center">00 : 00 : 15</td><td class="align_center">00 : 00 : 30</td></tr><tr class="table-tr"><td colspan="5"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>RT-qPCR conditions applied in the present study.</div>

BioMed Research International

tab2

Table 2

Table 2: Comparison of Primer-Probe Sets among Different Master Mixes for Laboratory Screening of Severe Acute Respiratory Syndrome Coronavirus 2 (<i>SARS-CoV-2</i>) 

Table 2 | Comparison of Primer-Probe Sets among Different Master Mixes for Laboratory Screening of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) 