Comparison of Primer-Probe Sets among Different Master Mixes for Laboratory Screening of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Table 3

The results of LightCycler Multiplex RNA Virus Master with three primer-probe sets (IDT, Phu Sa, and TIB-Molbiol).

Dilution	Viral copies	TIB-Molbiol; values			IDT; values			Phu Sa; values
		Day 1	Day 2	Day 3	Day 1	Day 2	Day 3	Day 1	Day 2	Day 3
1 : 10^7	96	34.47	34.22	35.11	34.82	34.22	35.18	34.51	34.91	34.93
		33.87	34.38	35.30	35.28	34.66	34.53	34.62	34.67	34.63
		34.50	34.75	35.14	35.25	34.49	34.85	35.12	34.74	34.85
1 : 10^7½	48	36.03	36.64	36.25	36.16	36.68	35.72	36.13	36.43	36.12
		36.81	35.35	35.87	36.32	35.97	36.35	36.62	36.17	36.35
		35.72	35.87	36.61	36.78	36.13	36.76	36.45	36.24	36.01
1 : 10^8	9.6	38.30	38.11	37.47	37.49	37.85	38.25	37.92	37.51	37.75
		36.87	38.14	37.38	37.82	38.28	37.22	37.63	37.93	38.22
		37.22	37.5	37.75	37.66	37.53	38.18	37.84	37.67	37.62
1 : 10^8½	4.8	39.25	38.37	39.03	39.35	39.58	39.24	39.12	39.24	39.21
		38.88	38.72	38.97	39.16	39.03	38.62	39.13	39.15	39.32
		39.24	39.50	39.21	39.25	38.99	39.28	39.17	39.33	39.41
Negative control		-	-	-	-	-	-	-	-	-
Positive control		22.33	25.31	25.60	30.0	25.4	28.18	26.37	29.48	26.37

“-”: undetected.