Knockdown of KCNQ1OT1 Inhibits Proliferation, Invasion, and Drug Resistance by Regulating miR-129-5p-Mediated LARP1 in Osteosarcoma

Figure 1

KCNQ1OT1 is highly expressed in osteosarcoma tissues and cells, and KCNQ1OT1-siRNA inhibits the proliferation, invasion, and drug resistance of osteosarcoma cells. (a) The relative level of KCNQ1OT1 mRNA in adjacent and osteosarcoma tumor tissues. (b) The expression of KCNQ1OT1 in osteoblasts (HFOB1.19) and osteosarcoma cells (HOS, MG63, 143B, and U20S). U20S cells were transfected with the control siRNA (Ctrl-siRNA group) or KCNQ1OT1-siRNA, respectively. (c) The relative mRNA expression of KCNQ1OT1 in the Ctrl-siRNA and KCNQ1OT1-siRNA groups. (d) The cell proliferation in the Ctrl-siRNA and KCNQ1OT1-siRNA groups. (e) The percentage of cell invasion in the Ctrl-siRNA and KCNQ1OT1-siRNA groups. (f) The IC50 for 5-Fu was calculated in the Ctrl-siRNA and KCNQ1OT1-siRNA group by MTT. (g) The protein expression of P-gp and MRP1 in the Ctrl-siRNA and KCNQ1OT1-siRNA groups. Compared with the HFOB1.19 or Ctrl-siRNA group at and compared with the adjacent group at . GAPDH was used as an invariant internal control for calculating protein fold changes.

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