Knockdown of KCNQ1OT1 Inhibits Proliferation, Invasion, and Drug Resistance by Regulating miR-129-5p-Mediated LARP1 in Osteosarcoma

Figure 2

KCNQ1OT1 binds to miR-129-5p directly, and the overexpression of LARP1 promotes proliferation invasion and drug resistance of osteosarcoma cells. (a) The binding region of KCNQ1OT1 and miR-129-5p predicted by StarBase. (b) Dual luciferase reporter assay was performed to measure the activity of determination of binding activity of KCNQ1OT1 and miR-129-5p. (c) Pull-down assay was performed to measure the binding activity of KCNQ1OT1 and miR-129-5p. (d) The relative expression of miR-129-5p in the NC and siKCNQ1OT1 groups. U20S cells were transfected with the control vector (vector group) or pcDNA3.1-LARP1 (LARP1 group), respectively. (e) The relative mRNA expression of LARP1 in the vector and LARP1 groups. (f) The cell proliferation in the vector and LARP1 groups in U20S cells. (g) The percentage of cell invasion in the vector and LARP1 groups. (h) The protein expression of P-gp and MRP1 in the vector and LARP1 groups. Compared with the control group at and ^#compared with the vector group at . Compared with the WT-KCNQ1OT1 or NC group at and compared with the control primer group at . “Special primer” is the specific primer of miR-129-5p, and “control primer” is the control group. GAPDH was used as an invariant internal control for calculating protein fold changes.

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