THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways

Figure 5

Overexpression of c-Myc rescued THAP11-induced cell growth inhibition. MKN-45 cells were transfected with both ov-THAP11 and ov-c-Myc, single ov-THAP11 or control. (a) c-Myc mRNA level was analyzed by qRT-PCR at 24 h after transfection. (b) MTT assay was used to detect the proliferation of cells at 24 h, 48 h, and 72 h after transfection, respectively. (c) Cell number was counted at 24 h, 48 h, and 72 h, respectively. (d) Cell cycle after was detected by flow cytometry at 24 h after transfection. The graph shows the percentage of cells in each phase. (e) Cell apoptosis was analyzed using the Annexin V-FITC apoptosis detection kit at 48 h after transfection. The graph shows the early and late apoptosis of cells. GAPDH was used as an internal control. (f) Expressions in c-Myc and its target genes in transfected cells were analyzed by Western blot at 48 h after transfection. (g) The bar graph shows the results of relative quantitative analysis of protein expression. GAPDH was used as an internal control. Data are shown as . Compared with control, ; compared with ov-THAP11, ^#, one-way ANOVA.

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