HAND2-AS1 Works as a ceRNA of miR-3118 to Suppress Proliferation and Migration in Breast Cancer by Upregulating PHLPP2

Figure 2

miR-3118 depletion inhibited BC proliferation while facilitating apoptosis in BC. (a) FISH assays were constructed to judge the subcellular localization of HAND2-AS1. (b) pcDNA3.1/HAND2-AS1 were transfected into cells, and the expression of miRNAs was detected in MCF-7 and SK-BR-3 cells. (c) The binding sequences between HAND2-AS1 and miR-3118 were projected by bioinformatics. (d) RNA pull-down assays were carried out to illustrate the combination between miR-3118 and HAND2-AS1. (e) miR-3118 mimics were transfected into cells, and the expression of miR-3118 was tested by RT-qPCR assays. (f) Luciferase reporter assays were conducted to demonstrate the combination between HAND2-AS1 and miR-3118. (g) The expression of miR-3118 was examined in BC cell lines by RT-qPCR assays. (h) miR-3118 expression was tested in cells transfected with miR-3118 inhibitor. (i, j) CCK8 and colony formation assays were applied to detect cell proliferation ability. (k) Flow cytometry analysis was conducted to probe the apoptosis rate. (l, m) Transwell assays were performed to estimate capacities of migration and invasion. (n) Western blot assays were conducted to measure associated proteins of the EMT process.

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