HAND2-AS1 Works as a ceRNA of miR-3118 to Suppress Proliferation and Migration in Breast Cancer by Upregulating PHLPP2

Figure 5

HAND2-AS1 hindered the course of BC through elevating PHLPP2 expression. (a) PHLPP2 expression was evaluated in cells transfected with pcDNA3.1/PHLPP2. (b, c) CCK8 and colony formation assays were carried out to estimate cell proliferation in cells transfected with pcDNA3.1/PHLPP2 and sh-PHLPP2. (d) The apoptosis rate was measured in flow cytometry analysis. (e, f) Transwell assays were conducted to evaluate capacity of migration and invasion. (g) Western blot was performed to measure relevant proteins of the EMT process.

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