Research Article
Toxicokinetics of 11 Gelsemium Alkaloids in Rats by UPLC-MS/MS
Table 3
Accuracy, precision, extraction efficiency, and matrix effects of 11
Gelsemium alkaloids in rat plasma (
).
| Compound | Concentration (ng/mL) | Accuracy (%) | CV (%) | Matrix effect (%) | Recovery (%) | Intraday | Interday | Intraday | Interday |
| Humantenirine | 0.1 | 103.2 | 98.4 | 5.7 | 9.9 | 95.4 | 84.0 | 0.4 | 95.5 | 95.4 | 7.1 | 13.7 | 93.6 | 81.8 | 18 | 99.4 | 99.2 | 3.0 | 7.5 | 97.3 | 89.9 | 180 | 99.5 | 107.4 | 7.7 | 8.8 | 93.6 | 86.8 | Humantenine | 0.1 | 98.4 | 104.7 | 7.3 | 13.0 | 99.1 | 85.8 | 0.4 | 97.7 | 98.3 | 2.1 | 5.2 | 98.8 | 89.1 | 18 | 102.0 | 100.8 | 3.7 | 5.0 | 103.6 | 86.0 | 180 | 96.5 | 106.3 | 4.7 | 8.4 | 98.1 | 90.0 | Akuammidine | 0.1 | 107.9 | 100.8 | 5.9 | 12.7 | 103.4 | 98.4 | 0.4 | 107.3 | 101.7 | 8.0 | 6.7 | 94.1 | 95.4 | 18 | 104.3 | 98.0 | 5.2 | 4.5 | 98.0 | 90.5 | 180 | 96.3 | 99.7 | 5.0 | 4.4 | 107.2 | 98.3 | Gelsevirine | 0.1 | 106.5 | 109.2 | 5.7 | 12.3 | 98.5 | 89.7 | 0.4 | 101.1 | 99.5 | 3.2 | 6.5 | 96.6 | 88.9 | 18 | 99.3 | 95.6 | 5.8 | 9.6 | 99.5 | 93.2 | 180 | 102.2 | 103.8 | 7.0 | 6.4 | 101.3 | 96.6 | Rankinidine | 0.1 | 99.3 | 101.5 | 14.9 | 13.3 | 103.2 | 93.8 | 0.4 | 102.2 | 97.9 | 7.8 | 2.2 | 104.2 | 91.0 | 18 | 103.2 | 105.0 | 8.8 | 12.6 | 107.7 | 86.5 | 180 | 97.4 | 101.6 | 7.1 | 5.8 | 103.2 | 92.0 | n-Methoxyanhydrovobasinediol | 0.1 | 92.7 | 100.9 | 5.0 | 8.3 | 98.8 | 95.9 | 0.4 | 106.0 | 97.9 | 4.0 | 4.5 | 97.7 | 89.0 | 18 | 102.7 | 94.9 | 3.4 | 6.2 | 105.4 | 90.9 | 180 | 97.4 | 103.8 | 2.5 | 4.0 | 103.4 | 90.6 | Gelsenicine | 0.1 | 104.5 | 98.2 | 10.9 | 13.8 | 90.6 | 91.6 | 0.4 | 102.8 | 103.5 | 4.0 | 12.3 | 88.5 | 84.3 | 18 | 105.1 | 97.0 | 3.3 | 5.3 | 95.1 | 82.2 | 180 | 92.5 | 92.2 | 6.9 | 5.4 | 98.9 | 93.5 | Gelsemine | 0.1 | 86.9 | 96.7 | 16.7 | 12.1 | 97.2 | 82.5 | 0.4 | 98.9 | 89.9 | 11.3 | 12.7 | 103.8 | 90.1 | 18 | 106.5 | 91.2 | 13.7 | 8.8 | 107.8 | 86.0 | 180 | 92.8 | 102.2 | 8.2 | 11.2 | 91.2 | 84.9 | Koumine | 0.1 | 113.2 | 91.8 | 6.9 | 15.9 | 94.2 | 95.6 | 0.4 | 108.3 | 99.6 | 11.8 | 8.1 | 92.8 | 83.3 | 18 | 99.6 | 91.7 | 8.3 | 11.2 | 91.6 | 82.5 | 180 | 98.2 | 108.3 | 12.7 | 13.9 | 89.6 | 84.7 | Koumidine | 0.1 | 102.3 | 103.9 | 11.1 | 14.6 | 100.8 | 78.2 | 0.4 | 105.5 | 101.2 | 9.5 | 6.1 | 97.7 | 79.8 | 18 | 97.5 | 105.8 | 10.3 | 10.6 | 100.2 | 76.4 | 180 | 99.1 | 96.6 | 5.7 | 8.9 | 94.0 | 82.7 | Sempervirine | 0.1 | 103.0 | 94.2 | 13.8 | 11.7 | 95.9 | 84.7 | 0.4 | 96.5 | 104.3 | 6.4 | 8.8 | 97.9 | 77.4 | 18 | 103.1 | 97.8 | 5.8 | 4.3 | 98.2 | 75.8 | 180 | 101.0 | 96.7 | 9.7 | 4.3 | 106.0 | 85.6 |
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