Long Noncoding RNA NEAT1 Regulates TGF-β2-Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells through the miR-34a/Snail1 and miR-204/Zeb1 Pathways

Figure 1

TGF-β2 induced overexpression of EMT markers in primary LECs through a NEAT1-dependent mechanism. (a) The primary HLECs were treated with indicated concentration of TGF-β2 for 48 h. The expression of NEAT1 was assessed by qRT-PCR. compared with the group without TGF-β2. (b) The primary HLECs were treated with 5 ng/ml TGF-β2 in indicated time. The expression of NEAT1 was assessed by qRT-PCR. compared with the group without TGF-β2. (c) The expression of NEAT1 was detected by qRT-PCR. compared with the control group. (d–g) The primary HLECs were treated with TGF-β2 (5 ng/ml) for 48 h before incubation with 100 nM siNEAT1-1 or siNEAT1-2 or si-control for 24 h. compared with the normal group. ^# compared with the group with TGF-β2. (d) The expression of NEAT1 was detected by qRT-PCR. (e) The protein levels of E-cadherin and fibronectin were detected by Western blot analysis. (f) E-cadherin mRNA expression was measured by qRT-PCR. (g) Fibronectin mRNA levels were analyzed using qRT-PCR. (a–g) The data are presented as the of six independent experiments.

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