Long Noncoding RNA NEAT1 Regulates TGF-β2-Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells through the miR-34a/Snail1 and miR-204/Zeb1 Pathways

Figure 9

NEAT1/Snail1 and NEAT1/Zeb1 pathways are involved in TGF-β2-induced EMT of LECs. (a) E-cadherin and fibronectin protein levels in primary HLECs were determined by Western blot analysis after knockdown of NEAT1, Snail1, and Zeb1. (b) E-cadherin mRNA levels were determined by qRT-PCR in primary HLECs after knockdown of NEAT1, Snail1, and Zeb1. (c) Fibronectin mRNA expression was determined by qRT-PCR in primary HLECs after knockdown of NEAT1, Snail1, and Zeb1. (b, c) compared with the TGF-β2 group. (d) E-cadherin and fibronectin protein levels in primary HLECs were determined by Western blot analysis after overexpression of NEAT1 or knockdown of Snail1 or Zeb1. (e) E-cadherin mRNA levels were determined by qRT-PCR in primary HLECs after overexpression of NEAT1 or knockdown of Snail1 or Zeb1. (f) Fibronectin mRNA was determined by qRT-PCR in primary HLECs after overexpression of NEAT1 or knockdown of Snail1 or Zeb1. (e, f) compared with the pcDNA3.1-NEAT1 group. The error bars represent the of six independent experiments.

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