Differential Gene Expression Responding to Low Phosphate Stress in Leaves and Roots of Maize by cDNA-SRAP

Table 3

BLAST annotations of a total of 54 genes based on the 63 DEFs of leaves and roots in maize under low Pi stress categorized into 11 classes of differential expression patterns. The DEFs annotating the candidate genes in response to low Pi stress in maize are highlighted in bold.

DEF #	Length (bp)	Organism	Accession	Description	Functional category	Tissue	value	Maximum identity
Class 1: transient-induced expression
018	324	Zea mays	AAD20307.1	Copia-type pol polyprotein	Unclassified	Leaf (3 days)		94%
028	444	Oryza sativa	AAK13160.1	Putative esterase	Esterase	Leaf (5 days)		84%
093	201	Zea mays	NP_001150949.1	bZIP transcription factor ABI5	Transcription	Leaf (5 days)		100%
096	201	Zea mays	NP_001150949.1	bZIP transcription factor ABI5	Transcription	Leaf (3 days)		100%
116	198	Arabidopsis lyrata	XP_002866900.1	DNAJ heat shock family protein	Genetic information processing	Leaf (3 days)		79%
117	193	Glycine max	BAG09374.1	Peroxisomal hydroxypyruvate reductase	Metabolism	Leaf (3 days)		88%
206	424	Oryza sativa	BAA24573.1	DNA polymerase alpha catalytic subunit	Metabolism	Leaf (3 days)		84%
211	440	Arachis hypogaea	AEL30371.1	TIR-NBS-LRR type disease resistance protein	Organismal systems	Root (3 days)		65%
234	492	Zea mays	ACR36983.1	Unknown	Unclassified	Root (3 days)		64%
332	350	Zea mays	ACG29577.1	Cytochrome P450 CYP78A55	Metabolism	Leaf (3 days)		74%
Class 2: rapidly switched off
002	675	Zea mays	NP_001105935.1	Elongation factor alpha8	Genetic information processing	Leaf		99%
145	310	Arabidopsis thaliana	NP_567433.1	SRPBCC ligand-binding domain-containing protein	Unclassified	Root		51%
161	316	Zea mays	NP_001183823.1	Hypothetical protein LOC100502416	Unclassified	Leaf		59%
188	398	Zea mays	NP_001148699.1	Tyrosyl-tRNA synthetase	Genetic information processing	Root		100%
257	360	Zea mays	ACG45582.1	Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH1	Metabolism	Leaf		88%
Class 3: gradually switched-off expression
005	427	Zea mays	NP_001150729.1	Aspartic proteinase oryzasin-1	Signal transduction	Leaf		99%
006	356	Arabidopsis lyrata	XP_002877001.1	Hydroxyproline-rich glycoprotein family protein	Metabolism	Leaf		40%
026	696	Arabidopsis thaliana	AAM64166.1	Cleavage stimulation factor 77	Unclassified	Leaf		67%
031	341	Zea mays	AAP94585.1	Putative gag-pol precursor	Unclassified	Leaf		82%
076	353	Oryza sativa	ABA94145.1	Retrotransposon protein	Unclassified	Leaf		83%
085	331	Candidatus Liberibacter americanus	ACD87749.1	Glycerol kinase	Metabolism	Leaf		76%
094	201	Zea mays	NP_001150949.1	bZIP transcription factor ABI5	Transcription	Leaf		100%
243	307	Zea mays	AAD20307.1	Copia-type pol polyprotein	Unclassified	Root		89%
262	209	Oryza sativa	AAK13589.1	rRNA intron-encoded homing endonuclease	Genetic information processing	Leaf		88%
Class 4: induced expression
044	346	Zea mays	ACG29272.1	Hypothetical protein	Unclassified	Root		73%
104	425	Oryza sativa Japonica group	ABF97883.1	Retrotransposon protein	Unclassified	Root		77%
115	209	Zea mays	NP_043052.1	Photosystem II protein H	Photosynthesis	Leaf		84%
179	240	Zea mays	BAA22440.1	Fatty acid desaturase	Metabolism	Leaf		100%
194	273	Litchichinensis	ADZ76112.1	Actin	Metabolism	Leaf		98%
202	239	Zea mays	ACG41852.1	Pyruvate decarboxylase isozyme 1	Metabolism	Root		100%
221	477	Oryza sativa	ABF95989.1	Retrotransposon protein, putative, Ty3-gypsy subclass, expressed	Unclassified	Root		60%
267	209	Oryza sativa	AAK13589.1	rRNA intron-encoded homing endonuclease	Genetic information processing	Root		88%
Class 5: switched-off–induced expression
013	427	Zea mays	NP_001150729.1	Aspartic proteinase oryzasin-1	Signal transduction	Root		99%
054	494	Zea mays	ACG34068.1	KOB1	Unclassified	Root		72%
173	142	Photorhabdus asymbiotica	YP_003039426.1	Exonuclease V subunit gamma	Genetic information processing	Root		72%
223	343	Oryza sativa	AAL47203.1	Chromatin-remodeling factor CHD3	Transcription	Root		60%
239	382	Zea mays	AAL66757.1	Putative polyprotein	Unclassified	Root		88%
337	367	Zea mays	NP_001141422.1	Protease Prl C candidate 1	Unclassified	Root		91%
Class 7: down expression
101	201	Zea mays	NP_001150949.1	bZIP transcription factor ABI5	Transcription	Root		100%
Class 8: down-up expression
062	401	Triticum aestivum	CBH32516.1	Alpha-glucan phosphorylase	Metabolism	Leaf		92%
181	314	Zea mays	NP_001146965.1	Vacuolar ATPase subunit H protein	Transport	Root		97%
235	471	Arabidopsis thaliana	NP_565520.1	Violaxanthin de-epoxidase-related	Metabolism	Root		50%
Class 10: up expression
079	352	Oryza sativa	AAQ56518.1	Putative polyprotein	Unclassified	Root		77%
120	279	Arabidopsis lyrata	XP_002893119.1	Tubulin family protein	Transport	Root		67%
189	250	Zea mays	ABF67926.1	Milt 1 A putative polyprotein	Unclassified	Root		91%
205	447	Nicotiana tabacum	AAF33670.1	Cyclic nucleotide-gated calmodulin-binding ion channel	Signal transduction	Leaf		56%
293	364	Zea mays	AAL73444.1	Putative non-LTR retroelement reverse transcriptase	Genetic information processing	Root		61%
Class 11: up-down expression
112	279	Populus trichocarpa	XP_002329950.1	Tubulin gamma complex-associated protein	Signal transduction	Leaf		78%
114	279	Populus trichocarpa	XP_002329950.1	Tubulin gamma complex-associated protein	Signal transduction	Leaf		78%
139	209	Zea mays	NP_043052.1	Photosystem II protein H	Photosynthesis	Root		84%
176	314	Zea mays	NP_001146965.1	Vacuolar ATPase subunit H protein	Transport	Leaf		95%
186	412	Zea mays	AAN40029.1	Putative gag-pol precursor	Unclassified	Leaf		87%
231	471	Arabidopsis thaliana	NP_565520.1	Violaxanthin de-epoxidase-related	Metabolism	Leaf		50%
Class 12: up-down-up expression
268	209	Oryza sativa	AAK13589.1	rRNA intron-encoded homing endonuclease	Genetic information processing	Root		88%
334	425	Oryza sativa	ABA99740.2	Retrotransposon protein, putative, unclassified	Unclassified	Root		43%
Class 13: induced–switched-off expression
050	401	Zea mays	ACG34068.1	KOB1	Unclassified	Leaf		100%
075	407	Zea mays	ABF67934.1	Opie3 pol polyprotein	Unclassified	Leaf		70%
172	284	Arabidopsis thaliana	NP_564010.1	Uridylyltransferase-related protein	Transferase	Leaf		74%
209	489	Glycine max	ABB00038.1	Reverse transcriptase family member	Genetic information processing	Root		51%
214	479	Gossypium hirsutum	AAP43917.1	Integrase	Integrase	Leaf		77%
220	477	Zea mays	AAL59229.1	Gag-pol	Unclassified	Root		62%