Pharmacokinetic Study of Thirteen Ingredients after the Oral Administration of Flos Chrysanthemi Extract in Rats by UPLC-MS/MS

Table 3

Precision and accuracy of 13 analytes in rat plasma ().

Compounds	Spiked concentration (ng/mL)	Intraday			Interday
		Measured concentration (ng/mL)	Accuracy (RE, %)	Precision (RSD, %)	Measured concentration (ng/mL)	Accuracy (RE, %)	Precision (RSD, %)
Luteolin	20		0.0	2.0		2.0	1.0
	200		-6.2	0.9		-5.8	1.3
	4000		2.1	1.1		2.8	1.6
Cynaroside	20		4.5	4.3		2.5	3.9
	200		4.1	1.0		0.5	0.4
	4000		-2.4	1.5		-2.0	1.0
Luteolin 7-O-glucuronide	10		-10.0	5.6		-10.0	4.5
	100		-11.5	0.7		-10.9	2.4
	2000		-8.0	1.5		-7.6	2.0
Isochlorogenic acid C	5		-8.0	4.3		-2.0	6.1
	50		-11.2	2.3		-9.8	2.9
	1000		2.0	2.6		2.0	3.4
Cryptochlorogenic acid	5		0.0	4.0		0.0	2.0
	50		-12.0	2.0		-11.6	1.8
	1000		3.1	2.3		3.2	2.4
Chlorogenic acid	5		-2.0	6.1		0.0	6.0
	50		-6.6	4.7		-5.4	4.7
	1000		5.8	2.0		7.5	2.6
Apigenin	5		12.0	1.8		12.0	1.8
	50		-11.0	2.7		-9.4	2.6
	1000		4.4	3.4		4.9	2.9
Acacetin	5		10.0	3.6		-2.0	12.2
	50		-9.8	1.6		-9.4	2.4
	1000		3.8	2.5		4.5	2.3
Hyperoside	5		-8.0	2.2		-6.0	4.3
	50		-8.4	2.4		-8.2	2.8
	1000		2.0	4.6		3.2	4.0
Isoquercitrin	5		-4.0	4.2		-2.0	4.1
	50		-10.4	1.1		-9.0	2.4
	1000		-5.5	4.4		0.1	3.0
Apigenin 7-glucoside	5		14.0	1.8		14.0	1.8
	50		-8.8	2.2		-9.0	2.2
	1000		7.8	2.5		7.0	3.2
Tilianin	1		-10.0	11.1		-10.0	11.1
	10		-3.0	4.1		-2.0	4.1
	200		0.8	1.2		0.3	1.9
Hesperidin	1		10.0	9.1		10.0	9.1
	10		-13.0	2.3		-11.0	2.2
	200		11.8	0.9		11.2	0.9